控制菌检验用培养基

心中的远方

固体培养基适用性检查促生长实验需要和对照培养基算回收率吗？比如麦康凯琼脂培养基，需要算回收率吗？

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落泪的蝴蝶

固体培养基促生长能力检查  用涂布法分别接种不大于100cfu的试验菌（表1）于被检培养基和对照培养基平板上，在相应控制菌检查规定的培养温度及不大于规定的最短培养时间下培养，被检培养基与对照培养基上生长的菌落大小、形态特征应一致。

Jpz77n2yf

控制菌检查用培养基的适用性无需进行回收率的计算，与对照培养基进行菌落大小以及形态特征的对比，可以多看看中国药典1106的培养基适用性检查。

心中的远方

落泪的蝴蝶 发表于 2024-07-30 14:39
固体培养基促生长能力检查  用涂布法分别接种不大于100cfu的试验菌（表1）于被检培养基和对照培养基平板上，在相应控制菌检查规定的培养温度及不大于规定的最短培养时间下培养，被检培养基与对照培养基上生长的菌落大小、形态特征应一致。

老师，美国药典要求，固体培养基适用性检查怎么做啊？

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心中的远方

Jpz77n2yf 发表于 2024-07-30 15:42
控制菌检查用培养基的适用性无需进行回收率的计算，与对照培养基进行菌落大小以及形态特征的对比，可以多看看中国药典1106的培养基适用性检查。

老师，美国药典要求，固体培养基适用性检查怎么做啊？

来自安卓APP客户端

心中的远方

落泪的蝴蝶 发表于 2024-07-30 14:39
固体培养基促生长能力检查  用涂布法分别接种不大于100cfu的试验菌（表1）于被检培养基和对照培养基平板上，在相应控制菌检查规定的培养温度及不大于规定的最短培养时间下培养，被检培养基与对照培养基上生长的菌落大小、形态特征应一致。

老师，美国药典要求，固体培养基适用性检查怎么做啊？

来自安卓APP客户端

落泪的蝴蝶

Growth Promotion of the Media
Test each batch of ready-prepared medium and each batch of medium prepared either from dehydrated medium or from
the ingredients described.
Inoculate portions/plates of Soybean–Casein Digest Broth and Soybean–Casein Digest Agar with a small number (not more
than 100 cfu) of the microorganisms indicated in Table 1, using a separate portion/plate of medium for each. Inoculate plates
of Sabouraud Dextrose Agar with a small number (not more than 100 cfu) of the microorganisms indicated in Table 1, using a
separate plate of medium for each. Incubate according to the conditions described in Table 1.
For solid media, growth obtained must not differ by a factor greater than 2 from the calculated value for a standardized
inoculum. For a freshly prepared inoculum, growth of the microorganisms comparable to that previously obtained with a
previously tested and approved batch of medium occurs. Liquid media are suitable if clearly visible growth of the microorganisms
comparable to that previously obtained with a previously tested and approved batch of medium occurs.这是美国药典对微生物限度培养基适用性要求

ceq518

没听说适用性检查要算回收率，看下指南怎么啦

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八档丶电风扇

固体培养基适用性不是要计算被检与对照的比值吗？控制菌检查琼脂培养基除了指示特性还要做促生长能力的，只要做促生长能力，都要计算回收率，你仔细看看1105和1106，都有写的

Rabbi

八档丶电风扇 发表于 2024-08-08 11:47
固体培养基适用性不是要计算被检与对照的比值吗？控制菌检查琼脂培养基除了指示特性还要做促生长能力的，只要做促生长能力，都要计算回收率，你仔细看看1105和1106，都有写的

1106没写了促生长能力要做回收率，只看到对加菌量有规定

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心中的远方

落泪的蝴蝶 发表于 2024-08-08 10:22
本帖最后由 落泪的蝴蝶 于 2024-8-8 10:25 编辑

Growth Promotion of the Media
Test each batch of ready-prepared medium and each batch of medium prepared either from dehydrated medium or from
the ingredients described.
Inoculate portions/plates of Soybean–Casein Digest Broth and Soybean–Casein Digest Agar with a small number (not more
than 100 cfu) of the microorganisms indicated in Table 1, using a separate portion/plate of medium for each. Inoculate plates
of Sabouraud Dextrose Agar with a small number (not more than 100 cfu) of the microorganisms indicated in Table 1, using a
separate plate of medium for each. Incubate according to the conditions described in Table 1.
For solid media, growth obtained must not differ by a factor greater than 2 from the calculated value for a standardized
inoculum. For a freshly prepared inoculum, growth of the microorganisms comparable to that previously obtained with a
previously tested and approved batch of medium occurs. Liquid media are suitable if clearly visible growth of the microorganisms
comparable to that previously obtained with a previously tested and approved batch of medium occurs.这是美国药典对微生物限度培养基适用性要求

老师，美国药典微生物限度方法学验证不用做供试品对照组吗？

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落泪的蝴蝶

心中的远方 发表于 2024-8-9 18:08
老师，美国药典微生物限度方法学验证不用做供试品对照组吗？

不做供试液对照，怎么计算回收率呢？

Rabbi

心中的远方 发表于 2024-08-09 18:08
老师，美国药典微生物限度方法学验证不用做供试品对照组吗？

USP里面没写就可以不做

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〆青春续写了幼

心中的远方 发表于 2024-8-9 18:08
老师，美国药典微生物限度方法学验证不用做供试品对照组吗？

供试品对照组是必须做的

心中的远方

〆青春续写了幼 发表于 2024-08-14 16:55
供试品对照组是必须做的

为什么呀？USP没写要做供试品对照组，它写的是只计算所加试验菌，回收率就是试验组：菌液对照组

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心中的远方

落泪的蝴蝶 发表于 2024-08-13 11:25
不做供试液对照，怎么计算回收率呢？

回收率就是试验组比菌液对照组

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