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EN13697上要求孢子状态是Spiny,请问具体是什么状态呢?
Carry out a microscopic examination under x 400 magnification immediately after the preparation to show:
— the presence of a high concentration (at least 75 % of spiny spores) of characteristic mature spores, i.e.
spiny spores (versus smooth spores);
— the absence of spore germination (check at least 10 fields of view);
— the absence of mycelia fragments (check at least 10 fields of view).
If germinated spores are present, discard the suspension.
If mycelia are present, proceed to a 2nd fritted filtration.
If mycelia are still present, discard the suspension.
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